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IF0100G Rickettsia IFA IgG
Indirect immunofluorescent antibody (IFA) test for the detection and semi-quantitation of human serum IgG antibodies to Rickettsia rickettsii, the etiologic agent of Rocky Mountain spotted fever, and to Rickettsia typhi, the etiologic agent of murine typhus. Each kit contains ten 8-well slides with individual spots of R. typhi and R. rickettsii in each well, yolk sac diluent, IgG conjugate, and positive and negative controls.
 
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Rickettsia IFA (IF0100G and IF0100M)

Slide 1 Slide 2
Negative Positive
Rickettsia Spotted Fever Rickettsia Typhus Fever
rickneg.jpg (29715 bytes) RickSpottedFeverGrpPOS.jpg (43110 bytes) RickTyphusGrpPOS.jpg (39404 bytes)
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Rickettsia IFA Slide Schematic


Rickettsia as Viewed through Microscope

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Frequently Asked Questions (FAQ)

Q: How are the antigens arranged on the slide?

A: Please see the slide schematic shown above for this information.

Q: Why is the positive control for this IFA kit barely positive at the screening dilution?

A: The positive controls are bottled at the screening dilution and can be titered for an endpoint from this dilution. The control should be used in the first well without further dilution.

Q: What antigens do you use for Rickettsia testing?

A: We have IFA slides with both Rickettsia rickettsii, representing the spotted fever group, and Rickettsia typhi representing the typhus fever group.

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Rickettsia IFA IgG
       
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